Filter by: Select category from list What does HEK mean? Hek From other capitalisation: This is a redirect from a title with another method of capitalisation. It leads to the title in accordance with the Wikipedia naming conventions for capitalisation, or it leads to a title that is associated in some way with the conventional capitalisation of this redirect title.
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Add it HERE! Still can't find the acronym definition you were looking for? Use our Power Search technology to look for more unique definitions from across the web! Search the web. Citation Use the citation options below to add these abbreviations to your bibliography. Powered by CITE. Get instant explanation for any acronym or abbreviation that hits you anywhere on the web! Download Close. Rate it:. Human Embryonic Kidney Medical.Suggest new definition. References in periodicals archive?
With a growing rental market in Saudi Arabia and the Middle East, we are delighted to work with Alimak Hek offering customised rental solutions. Byrne leads rental equipment market. Medicinal herbs Oerianthe javanica Blume DC. Moench protect human cells from [MPP. HEK [BK. Ca] cells transfected with ANXA5 siRNA see above were plated into well white walled plates, in triplicate for each experimental condition.
Functional Interactions between [BK. Antiproliferative evaluation of isofuranodiene on breast and prostate cancer cell lines.
In the present study, we investigated the neuroprotective effect of conditioned medium CM derived from human embryonic kidney HEK cells in a kainic acid KA induced hippocampal degenerationmodel system in in vitro condition. Conditioned medium reconditions hippocampal neurons against Kainic acid induced excitotoxicity: An in vitro study. Micropatterning of ECM proteins on glass substrates to regulate cell attachment and proliferation.
HEK shares advanced 2. Acronyms browser? Full browser?DNA transposons have emerged as indispensible tools for manipulating vertebrate genomes with applications ranging from insertional mutagenesis and transgenesis to gene therapy.
To fully explore the potential of two highly active DNA transposons, piggyBac and Tol2as mammalian genetic tools, we have conducted a side-by-side comparison of the two transposon systems in the same setting to evaluate their advantages and disadvantages for use in gene therapy and gene discovery. We have observed that 1 the Tol2 transposase but not piggyBac is highly sensitive to molecular engineering; 2 the piggyBac donor with only the 40 bp 3'-and 67 bp 5'-terminal repeat domain is sufficient for effective transposition; and 3 a small amount of piggyBac transposases results in robust transposition suggesting the piggyBac transpospase is highly active.
Performing genome-wide target profiling on data sets obtained by retrieving chromosomal targeting sequences from individual clones, we have identified several piggyBac and Tol2 hotspots and observed that 4 piggyBac and Tol2 display a clear difference in targeting preferences in the human genome. Finally, we have observed that 5 only sites with a particular sequence context can be targeted by either piggyBac or Tol2.
The non-overlapping targeting preference of piggyBac and Tol2 makes them complementary research tools for manipulating mammalian genomes.
PiggyBac is the most promising transposon-based vector system for achieving site-specific targeting of therapeutic genes due to the flexibility of its transposase for being molecularly engineered. Insights from this study will provide a basis for engineering piggyBac transposases to achieve site-specific therapeutic gene targeting. DNA transposons are natural genetic elements residing in the genome as repetitive sequences.
A simple transposon is organized by terminal repeat domains TRDs embracing a gene encoding a catalytic protein, transposase, required for its relocation in the genome through a "cut-and-paste" mechanism. Since the first discovery of DNA transposons in Maize by Barbara McClintock in [ 1 ], transposons have been used extensively as genetic tools in invertebrates and in plants for transgenesis and insertional mutagenesis [ 2 - 7 ]. Since its awakening, Sleeping Beauty has been used as a tool for versatile genetic applications ranging from transgenesis to functional genomics and gene therapy in vertebrates including fish, frogs, mice, rats and humans [ 9 ].
Subsequently, naturally existing transposons, such as Tol2 and piggyBachave also been shown to effectively transpose in vertebrates. The Medaka fish Orizyas latipes Tol2belonging to the hAT family of transposons, is the first known naturally occurring active DNA transposon discovered in vertebrate genomes [ 10 ].
Tol2 is a standard tool for manipulating zebrafish genomes and has been demonstrated to transpose effectively in frog, chicken, mouse and human cells as well [ 11 ]. Recent studies found that Tol2 is an effective tool both for transgenesis via pronuclear microinjection and germline insertional mutagenesis in mice [ 12 ].
Cabbage looper moth Trichoplusia ni piggyBac is the founder of the piggyBac superfamily and is widely used for mutagenesis and transgenesis in insects [ 13 ]. Recently, piggyBac was shown to be highly active in mouse and human cells and has emerged as a promising vector system for chromosomal integration, including insertional mutagenesis in mice and nuclear reprogramming of mouse fibroblasts to induced-pluripotent stem cells [ 14 - 19 ].
To date, most gene therapy trials have utilized viral vectors for permanent gene transfer due to their high transduction rate and their ability to integrate therapeutic genes into host genomes for stable expression. However, serious problems associated with most viral vectors, such as limited cargo capacity, host immune response, and oncogenic insertions as evidenced by the retrovirus-based gene therapy highlight an urgent need for developing effective non-viral therapeutic gene delivery systems [ 2021 ].
Recently, Sleeping BeautyTol2and piggyBac transposon-based vector systems have been explored for their potential use in gene therapy with proven successes [ 22 - 25 ].As a world leading biotech products provider, Creative Bioarray produces the world's most comprehensive list of research-use cells, including tumor cells, primary cells, stem cells and transformed cells.
Since its foundation inthe company has updated its cell products list for many times, along with its extension of business market and increasing annual sales. The hek cells are very easy to work with, which means they can be straightforwardly grow in culture and to transfect, and are very popular as hosts for gene expression. It can be used in many applications such as these experiments involve transfecting in a gene of interest, and then analyzing the expressed protein.
This specific cell line are originally derived from human embryonic kidney cells grown in tissue culture and from still born animals. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy. Propelled by integrity, expertise and the desire to simplify, improve, and accelerate research speed of the scientists, the company provides the highest standard quality research products and services including human and animal tissues, tissue array, microorganisms, cells, probe and cell services.
Feedback For webmasters. Periodicals Literature. Keyword Title Author Topic.Human embryonic kidney cellsalso often referred to as HEKHEKcellsor less precisely as HEK cellsare a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. HEK cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection.
They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy. HEK cells were generated in by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands.
The cells were obtained from a single, apparently healthy, legally aborted fetus under Dutch law; the identity of the parents and the reason for the abortion are unknown.
Graham performed the transfection a total of eight times, obtaining just one clone of cells that were cultured for several months. After presumably adapting to tissue culture, cells from this clone developed into the relatively stable HEK line.
For many years it was assumed that HEK cells were generated by transformation of either a fibroblasticendothelial or epithelial cellall of which are abundant in kidneys. However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK line may have been unusual in some fashion.
Graham and coworkers provided evidence that HEK cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neuronssuggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. A comprehensive study of the genomes and transcriptomes of HEK and five derivative cell lines compared the HEK transcriptome with that of human kidney, adrenal, pituitary and central nervous tissue.
Given the location of the adrenal gland adrenal means "next to the kidney"a few adrenal cells could plausibly have appeared in an embryonic kidney derived culture, and could be preferentially transformed by adenovirus. Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells.
As a consequence, HEK cells should not be used as an in vitro model of typical kidney cells. HEK cells have a complex karyotypeexhibiting two or more copies of each chromosome and with a modal chromosome number of They are described as hypotriploid, containing less than three times the number of chromosomes of a haploid human gamete. Chromosomal abnormalities include a total of three copies of the X chromosome and four copies of chromosome 17 and chromosome HEK cells are straightforward to grow in culture and to transfect.
They have been used as hosts for gene expression. Typically, these experiments involve transfecting in a gene or combination of genes of interest, and then analyzing the expressed protein.
A more specific use of HEK cells is in the propagation of adenoviral vectors. However, as pathogensthey also present a risk to the experimenter. This danger can be avoided by the use of viruses which lack key genes, and which are thus unable to replicate after entering a cell. In order to propagate such viral vectors, a cell line that expresses the missing genes is required. Since HEK cells express a number of adenoviral genes, they can be used to propagate adenoviral vectors in which these genes typically, E1 and E3 are deleted, such as AdEasy.
An important variant of this cell line is the T cell line. It contains the SV40 Large T-antigen that allows for episomal replication of transfected plasmids containing the SV40 origin of replication. This allows for amplification of transfected plasmids and extended temporal expression of desired gene products. Depending on various conditions, the gene expression of HEK cells may vary. The following proteins of interest among many others are commonly found in untreated HEK cells:.
From Wikipedia, the free encyclopedia. Redirected from HEK cell. Cell line derived from human embryonic kidney cells. Retrieved August 11, Nature Communications. Bibcode : NatCo Archived from the original on Retrieved Die logika wat gewoonlik uitgevoer word is Boolse logika en word algemeen aangetref in digitale stroombane. Omdat die afvoer ook 'n logiese toestand is, kan die afvoer van een logiese hek verbind word aan die toevoer van ander een of meer logiese hekke.
Twee afvoere kan egter nie aanmekaar verbind word nie, aangesien hulle moontlik kan probeer om verskillende logiese toestande te lewer. In elektroniese logiese hekke, sal dit lei tot 'n kortsluiting. Met elektroniese logika, word 'n logiese toestand verteenwoordig deur 'n sekere spanning wat sal afhang van die soort logika wat gebruik word.
Elke logiese hek vereis elektriese krag om die elektriese strome te manipuleer om die korrekte afvoerspannings te lewer. In logiese stroombaandiagramme word die elektriese krag nie aangetoon nie, maar in volledige elektroniese tekeninge moet die kragverbindings aangetoon word.
In elektroniese stroombane, word die spannings van die logiese toestande vasgestel. Dit is nie moontlik om enige ander sein deur die hekke te stuur nie, aangesien die logiese toestande die enigste moontlike spannings is. Vir meer inligting oor hoe moderne halfgeleier logiese hekke werk, sien CMOS. Die NEN-hek het inderwaarheid die laagste komponenttelling van enige hek buiten die NIE-hek wanneer dit met moderne halfgeleiertegnieke aanmekaargesit word en aangesien 'n NEN beide 'n NIE en, deur die toepassing van De Morgan se wet'n OF-funksie kan implementeer kan hierdie enkele hek effektief die EN- OF- en NIE-hekke vervang wat dit die enigste hek maak wat werklik in 'n stelsel benodig word.
Programmeerbare logiese matrikse sal dikwels niks anders as NEN-hekke bevat nie om sodoende hulle interne ontwerp te vereenvoudig. Die eerste voorbeeld is die EN-hekwaarvoor die waarheidstabel hieronder links aangedui word.
Die afvoer is 1 as beide toevoer A en toevoer B 1 is. In alle ander gevalle is die afvoer 0. In geskakelde stroombaan, is die stroombaan geslote wanneer beide A en B skakelaars aan is, anders is die stroombaan oop. Die afvoer is 1 wanneer die toevoer A of toevoer B 1 is.
Die afvoer is ook 1 as beide toevoere 1 is. In die skakelaarstroombaan, is die stroombaan geslote wanneer skakelaar A of skakelaar B of beide aangeskakel word, andersins is die stroombaan oop.
In die NIE-hek is die afvoer die logiese teenoorgestelde van die toevoer. Dit beteken dat as die toevoer 1 is, dat die afvoer 0 gaan wees en as die toevoer 0 is dat die afvoer 1 gaan wees. In die skakelaarstroombaan word 'n skakelaar gebruik wat normaalweg aangeskakel is om 'n geslote stroombaan te lewer. As die skakelaar gedruk word verander die stroom in 'n oop stroombaan. XOF is 'n 'strenger' weergawe van die OF-hek. In plaas van dat die afvoer 1 is wanneer enige van die toevoere 1 is, het die XOF-hek 'n 1 as afvoer wanneer slegs een van die toevoere 1 is, soos gesien kan word in die onderstaande waarheidstabel.
In die geval van 'n meervoudige toevoer XOF-hek sal die afvoer 1 wees wanneer daar 'n onewe getal 1'e as toevoer is. Dit kan ook gesien word as 'n 1 afvoer wanneer die toevoere gelyk is. Die voorafgaande eenvoudige logiese hekke kan gekombineer word om meer komplekse Boolse logikastroombane te vorm. Skakelaar stroombaandiagram vir die EN-hek. Skakelaar stroombaandiagram vir die OF-hek. Skakelaar stroombaandiagram vir die NIE-hek. Skakelaar stroombaandiagram van 'n NEN-hek.
Skakelaar stroombaandiagram van die NOF-hek. Kategorie : Elektronika. Naamruimtes Bladsy Bespreking. Weergawes Lees Wysig Wysig bron Wys geskiedenis.
Wikimedia Commons.Jump to navigation. TLR8 was initially thought to be non-functional in mice . Of note, this does not hold true when using TL, an analog of the synthetic agonist VX Thus, findings regarding mouse TLR8 are not transposable to its human counterpart [2,4]. Structural analyses have revealed that TLR8 possesses two binding sites with distinct specificities.
Georg P. Innate sensors that regulate vaccine responses. Heil F. Species-specific recognition of single-stranded RNA via Toll-like receptor 7 and 8. Choo M. TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects. Eigenbrod T. Bacterial RNA: an underestimated stimulus for innate immune responses.
Tanji H. Toll-like receptor 8 senses degradation products of single-stranded RNA. Liu J. A five-amino-acid motif in the undefined region of the TLR8 ectodomain is required for species-specific ligand recognition. OD fold increase over non-induced cells is shown. Species-driven TLR8 differential responses.
Growth medium: DMEM, 4. Research Fields. Custom Services. Please contact our distributor. Add to favorite. References: 1. The stability for 20 passages, following thawing, has been verified.
HEK 293 cells
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